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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Signature of circular RNAs in human induced pluripotent stem cells and derived cardiomyocytes

Fig. 1

Identification of hiPSCs and hiPSC-derived cardiomyocytes. a Representative photographs of fibroblasts on day 6 and human fibroblast-derived iPSC colonies on day 30 during hiPSC generation, as well as passaged hiPSCs under bright field at 100× magnification. b Immunofluorescence staining of hiPSCs for pluripotency markers including NANOG, SOX2 and TRA-1-60, and nucleus marker DAPI. c Immunofluorescence staining of hiPSC-derived cell for markers of endoderm (SOX17), mesoderm (SMC), ectoderm (TUJ1) and nucleus (DAPI). d Hematoxylin and eosin staining of hiPSC-derived teratoma comprising of epithelium, cartilage and neuro (asterisks). e Immunofluorescence staining of hiPSC-CMs for cardiac markers including α-SA and TNNT2, and nucleus marker DAPI. f Line-scan images and corresponding spontaneous Ca2+ transients in hiPSC-CMs. Ca2+ level calculated as ΔF/F0 = (F – F0) / F0, where F0 is diastolic fluorescence level. iPSC induced pluripotent stem cell

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