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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Signature of circular RNAs in human induced pluripotent stem cells and derived cardiomyocytes

Fig. 3

Cell-specific expression of circRNAs in hiPSCs and hiPSC-CMs. a Proportion of circRNA reads among total mapped reads higher in the hiPSC-CM group (CM) than in the hiPSC group (iPSC). b RNA-seq data showed increased expression of Quaking (QKI) and RNA binding Motif protein 20 (RBM20), two alternative factors associated with circRNA formation. c qRT-PCR detection of QKI and RBM20 mRNA in hiPSCs and hiPSC-CMs; data shown as mean ± SD, Student’s t test, *p < 0.05. d Hierarchical clustering (heat map) showed differential expression of circRNAs among hiPSCs and hiPSC-CMs. e Confirmation of differentially expressed circRNAs by RT-PCR. f Representative electrophoretograms of RT-PCR showed differential expression of circRNAs from days 0 to 30 during cardiac differentiation from hiPSCs. g Schematic depiction of circCACNA1D generation by back-splicing of exon 3 to exon 2, and verification of back-splicing site of circCACNA1D by Sanger sequencing. h qRT-PCR detection of QKI mRNA in negative control (NC) and siQKI transfected hiPSC-CMs. i qRT-PCR showed decreased expression of circRNAs in siQKI transfected hiPSC-CMs, compared with that in NC. circRNA circular RNA, CM cardiomyocyte, ESC embryonic stem cell, FB fibroblast, iPSC induced pluripotent stem cell. Data in h and i shown as mean ± SD, one-way ANOVA followed by Turkey’s test, *p < 0.05

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