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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails

Fig. 1

Optimization of concentration and duration of CHIR99021 treatment during DE induction. qRT-PCR for indicated genes using RNA lysates from human iPSCs treated with CHIR99021 at 1 μM or 3 μM for 24, 48, and 72 h during differentiation. Relevant expression of markers for pluripotency (a), DE (b), mesoderm (c) and ectoderm (d) were showed. e qRT-PCR for pluripotent markers (OCT4, NANOG), DE markers (SOX17, FOXA2), mesoderm markers (HAND1, BRA), and ectoderm markers (GAP43, ZIC1) using RNA lysates from human iPSCs exposed to CHIR99021 continuously or intermittent for 48 h. Growth factor-based method (activin A) was used for comparison

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Stem Cell Research & Therapy

ISSN: 1757-6512