Fig. 5

Functional analysis of small-molecule-induced hepatocyte-like cells. a PAS staining showing glycogen storage in small-molecule- and growth factor-induced differentiated cells. Freshly isolated human primary hepatocytes (hPH) were used as the control. Scale bars = 100 μm. b Cytochrome P450 1A2 activity in small-molecule- (SM-iHep) and growth factor-induced hepatocytes (GF-iHep) after induction with omeprazole (1A2). c Western blotting for albumin expression of small-molecule-induced hepatocyte-like cells from hESCs-H1 and hiPSCs. GAPDH was used as the loading control. d Albumin secretion of the differentiated cells treated with small-molecule cocktails or growth factors. Undifferentiated human iPSCs and freshly isolated human primary hepatocytes (hPH) were used as controls. e Analysis of ICG uptake (left) and ICG release 6 h later (right) of small-molecule-induced hepatocyte-like cells. Scale bars = 100 μm. (^*p value < 0.05, ^**p value < 0.01)