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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails

Fig. 6

The established chemical differentiation protocol is also applicable to other human pluripotent stem cell lines. a qRT-PCR analysis of pluripotency and DE-specific markers in hESC-H1 differentiated cells at the endpoint of stage I. Undifferentiated hESC-H1 were used as control. b Immunofluorescence of FOXA2 and SOX17 in hESC-H1 differentiated cells at the endpoint of stage I. Scale bars = 50 μm. c Gene expression of hepatic progenitor cell markers in hESC-H1 differentiated cells at the endpoint of stage II. Undifferentiated hESC-H1 cells were regarded as control. d Immunofluorescence of AFP and HNF4α in hESC-H1-differentiated cells at the endpoint of stage II. Scale bars = 50 μm. e qRT-PCR analysis of hepatocyte markers in hESC-H1-differentiated cells at the endpoint of stage III. Undifferentiated hESC-H1 and freshly isolated human primary hepatocytes (hPH) were regarded as controls. f Immunofluorescence of A1AT and ALB in hESC-H1-differentiated cells at the endpoint of stage III. Scale bars = 50 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512