Fig. 2

PRP affected cell cycle and apoptosis of MenSCs. a Flow cytometry analysis of cell cycle (n = 6) after treatment with 10% PRP or 10% FBS for 24 h. The percentage of cells in S stage was significantly higher in MenSCs of PRP group, demonstrating a DNA synthesis promotion. b Flow cytometry detection of cell apoptosis by Annexin V/PI staining. P4 MenSCs (n = 6) were treated for 24 h or 48 h then were tested. Early, late, and total apoptosis declined after PRP treatment for 24 h. Data was mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001 for two-tailed t test