Table 1 Primer sequences used for quantitative polymerase chain reaction analysis
Gene | Forward 5’ to 3’ | Reverse 5’ to 3’ |
|---|---|---|
GAPDH | CAAGGTCATCCATGACAACTTTG | GGCCATCCACAGTCTTCTGG |
ACTA | CTCGGAGATCATCACGTTTG | CCTTGGAAATCTCGAAGTGC |
LIN28 | GAAGCGCAGATCAAAAGGAG | GCTGATGCTCTGGCAGAAGT |
OCT4 | GCAAAACCCGGAGGAGTC | CCACATCGGCCTGTGTATATC |
SOX2 | TTGCTGCCTCTTTAAGACTAGGA | CTGGGGCTCAAACTTCTCTC |
NANOG | CCAACATCCTGAACCTCAGC | GCTATTCTTCGGCCAGTTG |
DPPA2 | TGGTGTCAACAACTCGGTTTG | CTCGAACATCGCTGTAATCTGG |
TGFb1 | GCAGCACGTGGAGCTGTA | CAGCCGGTTGCTGAGGTA |
PDGFRA | AGGTGGTTGACCTTCAATGG | TTTGATTTCTTCCAGCATTGTG |
FN1 | CTGGCCGAAAATACATTGTAAA | CCACAGTCGGGTCAGGAG |
hERVFRD | TGAGGAGGGCAATCCATTTCAT | TGCATGGTCGTTAAGGCTT |
hPAX9 | GTTATGTTGCTGGACATGGGT | GAAGCCGTGACAGAATGACTAC |