Fig. 1
From: Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells
Protocol design. Schematic diagram illustrating three steps for directed differentiation of undifferentiated hiPSCs into NP-like cells. E8 medium = Essential 8™ medium (STEMCELL Technologies, Seattle, WA, USA); hiPSC = human induced pluripotent stem cell; d = days; 6w = six-well; SHH = sonic hedgehog; T = brachyury; BMP4 = bone morphogenetic protein 4; FOXA2 = forkhead box protein A2; FGF2 = basic fibroblast growth factor; CD24 = cluster of differentiation 24 protein; BASP1 = brain abundant membrane attached signal protein 1; MIXL1 = paired-type homeobox transcription factor identified in human; CDX2 = member of the caudal-related homeobox transcription factor family; TGF = transforming growth factor; LMα5 = alpha-5 subunit of heterotrimeric laminin; NOG = noggin; NOTO = notochord homeobox