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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells

Fig. 2

Pluripotency for derived colonies confirmed via immunofluorescence. a Undifferentiated human iPSCs cultured in colonies on vitronectin-coated plates until induction of differentiation (scale bar = 50 μm). b Immunofluorescence showing DNA (green) and positive pluripotent marker expression (red) of TRA1–60, SOX2, OCT4, TRA1–81, and SSEA-4. Yellow indicates costaining of green nuclei and red pluripotent markers (scale bar = 50 μm)

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