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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection

Fig. 7

BCG induced iPS-Mφ apoptosis by flow cytometric analysis of FITC Annexin V staining. iPS-Mφ were treated with BCG for 24 h. FITC Annexin V and propidium iodide (PI)-positive cells were analyzed by flow cytometry. The majority (37.77 ± 7.94%, n = 3) of treated iPS-Mφ became PI positive, FITC Annexin V positive, or both FITC Annexin V and PI positive. In contrast, only a small portion (4.97 ± 1.60%) of untreated iPS-Mφ was PI positive, FITC Annexin V positive, or both FITC Annexin V and PI positive (P < 0.01). The portion of treated THP-1-Mφ was 37.1 ± 2.84% and untreated THP-1-Mφ was 6.19 ± 1.68%. Statistical analysis was performed by using the t test. A value of P < 0.01 represents a statistically significant difference (Upper panel: upper right quadrant refers to late apoptosis; upper left quadrant refers to necrotic cells; lower left quadrant refers to viable cells; lower right quadrant refers to early apoptosis). TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling. The apoptosis analysis was repeated at least three times and demonstrated consistent results. Note: the upper panel: a was untreated iPS-Mφ, b was treated iPS-Mφ, c was untreated THP-1-Mφ, d was treated THP-1 -Mφ; the lower panel was the ratio of the apoptosis

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