Table 1 Primer sequences for control and target genes and q-PCR conditions

TSG-6

S: GATGGATGGCTAAGGGCAGAGT-3′

93 °C, 20 s, 61 °C, 20 s 72 °C 30 s

208

NM_007115.3

Liu et al. 2016 [44]

AS: TCATTTGGGAAGCCTGGAGATT-3′

EP2

S: 5-CCACCTCATTCTCCTGGCTA-3

93 °C, 20 s, 62 °C, 20 s 72 °C 30 s

216

NM_000956.3

Kunisch et al. 2009 [45]

AS: 5-CGACAACAGAGGACTGAACG-3

HGF

S: 5′-CTCACACCCGCTGGGAGTAC-3′

93 °C, 20 s, 62 °C, 20 s 72 °C 30 s

104

XM_011516115.2

Jankowski et al. 2003 [46]

AS: 5′-TCCTTGACCTTGGATGCATTC-3′

RUNX2

S: GCCTTCAAGGTGGTAGCCC-3′

93 °C, 20 s, 62 °C, 20 s 72 °C 30 s

67

XM_017011396.1

Shafiee et al. 2011 [47]

AS: CGTTACCCGCCATGACAGTA-3′

OPN

S: 5′-TTGCAGCCTTCTCAGCCA-3′

93 °C, 20 s, 62 °C, 20 s 72 °C 30 s

76

NM_001251830.1

Bahrambeigi et al. 2012 [48]

AS: 5′-GGAGGCAAAAGCAAATCACTG-3′

FABP4

S: 5′-ATGGGATGGAAAATCAACCA-3′

93 °C, 20 s, 59 °C, 20 s 72 °C 30 s

87

NM_001442.2

Ponnaiyan et al. 2014 [49]

AS: 5′-GTGGAAGTGACGCCTTTCAT-3′

GAPDH

S: 5′-GACTCATGACCACAGTCCATGC-3′

93 °C, 20 s, 67 °C, 20 s 72 °C 30 s

112

NM_001289746.1

Liang et al. 2015 [50]

AS: 5′-AGAGGCAGGGATGATGTTCTG-3′

NANOG

S: 5′-CAAAGGCAAACAACCCACT-3′

93 °C, 30 s, 60 °C, 30 s 72 °C 30 s

427

NM_024865.2

Kozhukharova et al., 2009 [23]

AS: 5′-CTGGATGTTCTGGGTCTGGT-3′

OCT4

S:5′-AGCCCTCATTTCACCAGGCC-3′

93 °C, 30 s, 63 °C, 30 s 72 °C 30 s

456

NM_002701.5

Liedtke et al., 2007 [51]

AS:5′-TGGGACTCCTCCGGGTTTTG-3′

SOX2

S:5′ GCGCATGGACAGTTACGC-3′

93 °C, 30 s, 60 °C, 30 s 72 °C 30 s

276

NM_003106.3

Koshkin et al. 2016 [52]

AS: 5′ TCGGACTTGACCACCGAAC-3′

ACTIN

S:5′-GCCGAGCGGGAAATCGTGCGT-3′

93 °C, 30 s, 70 °C, 30 s 72 °C 30 s

507

NM_001101.3

Alekseenko et al., 2012 [53]

AS:-5’-CGGTGGACGATGGAGGGGCCG-3′