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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model

Fig. 1

Characterization of islet-1 human mesenchymal stem cells (ISL1-hMSCs). Flow cytometric analysis (a) showed that ISL1-hMSCs were positive for CD29, CD44, CD73, and CD90, and negative for CD14, CD31, CD34, and CD45. qPCR (b) showed that lentiviral transduction of hMSCs with ISL1 specifically increased the expression of ISL1. Flow cytometry (c) and immunofluorescence staining (d) further confirmed the overexpression of ISL1. Adipogenic differentiation of ISL1-hMSCs was determined by histochemical staining of adipocytes (Oil-Red O), osteogenic differentiation of ISL1-hMSCs was determined by histochemical staining of osteocytes (Alizarin Red), and chondrogenic differentiation was detected by Alcian Blue (e). Data are presented as the mean ± SD, n = 3; *p < 0.05 vs. Ctrl-hMSCs. Scale bar = 50 μm. Ctrl control, DAPI 4’6-diamidino-2-phenylindole

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