Fig. 5
Islet-1 (ISL1) overexpression increased human mesenchymal stem cell (hMSC) survival after transplantation of post-myocardial infection (MI) hearts. Representative images (a) and quantification (b) of surviving ISL1-hMSCs and control (Ctrl)-hMSCs in hearts at 7 days after transplantation. Red immunofluorescence of CM-Dil indicates surviving cells, and blue color of 4’6-diamidino-2-phenylindole (DAPI) indicates nuclei. Human nuclear antigen (HNA) staining is also shown. Representative flow cytometric images (c) with Annexin V/propidium iodide (PI) double staining assay of cells treated with or without H2O2. Quantification of Annexin-V-positive cells is shown as the apoptosis rate (d): (Annexin-V-positive cell amount / total cell amount) × 100%. Representative images and quantification (e) of cleaved caspase3 and full caspase3 in ISL1-hMSCs and Ctrl-hMSCs. Data are presented as the mean ± SD, n = 3; *p < 0.05 vs. Ctrl-hMSCs or H2O2 + Ctrl-hMSCs. Scale bar = 50 μm