Fig. 6
The anti-apoptotic effect of islet-1 human mesenchymal stem cells (ISL1-hMSCs) conditioned medium (CM) on cardiomyocyte cell line H9c2 subjected to oxidative injury (a) and quantification (b) of TUNEL staining in cardiomyocytes subjected to 200 μM H2O2 for 6 h. Cell nuclei were stained with 4’6-diamidino-2-phenylindole (DAPI; blue) and TUNEL-positive nuclei (green). Apoptosis rate = (TUNEL-positive nuclei / DAPI-positive nuclei) × 100%. Data are presented as the mean ± SD, n = 3; *p < 0.05 vs. control; #p < 0.05 vs. H2O2; &p < 0.05 vs. H2O2 + Ctrl-hMSCs. Scale bar = 100 μm. Ctrl control