Fig. 8
The anti-apoptotic effect of insulin-like growth factor binding protein 3 (IGFBP3) in islet-1 human mesenchymal stem cells (ISL1-hMSCs) conditioned medium (CM) on cardiomyocytes subjected to oxidative injury. Representative images of TUNEL staining in cardiomyocytes (a). Cell nuclei were stained with 4’6-diamidino-2-phenylindole (DAPI; blue) and TUNEL-positive nuclei (green). Quantification of TUNEL staining was shown. Apoptosis rate = (TUNEL positive nuclei / DAPI + nuclei) × 100% (b). Graphic of the mechanisms underlying the therapeutic effects of transplantation of ISL1-hMSCs (c). Data are presented as the mean ± SD, n = 3; *p < 0.05 vs. control; #p < 0.05 vs. H2O2; &p < 0.05 vs. H2O2 + Ctrl-hMSCs; @p < 0.05 vs. H2O2 + ISL1-hMSCs. Scale bar =100 μm. Ctrl control