Safranin O/Fast Green
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Surface/superficial architecture
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Cartilage defect
|
Adjacent
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Inflammatory cell infiltrate
|
Cartilage defect
|
Adjacent
|
Repair–host integration
|
Cartilage defect
|
Basal neocartilage–bone integration
|
Cartilage defect
|
Surface/superficial assessment
|
Cartilage defect
|
Adjacent
|
Mid/deep zone assessment
|
Cartilage defect
|
Adjacent
|
Overall assessment
|
Cartilage defect
|
Adjacent
|
Matrix staining (metachromasia)
|
Cartilage defect
|
Adjacent
|
Calcification/ossification
|
Cartilage defect
|
Adjacent
|
Subchondral bone vascularization
|
Bone subchondral
|
Surrounding
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Presence of bone void (with or without fibrovascular tissue and without secretory lining), yes/no
|
Bone subchondral
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Immunohistochemistry
|
C2: type II collagen
|
Cartilage defect
|
Adjacent
|