Fig. 1

LRRC15 is a potential target for osteoporosis treatment. a LRRC15 expression significantly induced when MSCs undergo osteogenic induction, determined by RT-qPCR and western blot analysis respectively. b Representative micro-CT and H&E staining of bone loss in OVX mice. Scale bars for μCT and H&E represent 400 μm and 50 μm respectively. c Bone volume, trabecular number, trabecular spacing, and trabecular thickness detected in Sham and OVX mice. d mRNA expression of Lrrc15 and protein levels of LRRC15 and RUNX2 tested in bone marrow-derived MSCs from OVX mice in contrast to Sham mice, determined by RT-qPCR (left), western blot (middle), and quantification (right) analysis. All data shown as mean ± SD, n = 3. **P < 0.01. w week, LRRC15 fifteen-leucine-rich repeat containing membrane protein, OCN osteocalcin, GAPDH glyceraldehyde-3-phosphate dehydrogenase, μCT microcomputed tomography, HE hematoxylin and eosin, OVX ovariectomized, Bv/Tv trabecular bone volume/tissue volume, Tb.N trabecular number, Tb.Sp trabecular spacing, Tb.Th trabecular thickness, RUNX2 runt-related transcription factor 2