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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: LRRC15 promotes osteogenic differentiation of mesenchymal stem cells by modulating p65 cytoplasmic/nuclear translocation

Fig. 2

Knockdown of LRRC15 inhibits osteogenic differentiation in vitro. a Left: Microscopic images of GFP-positive MSCs under ordinary and fluorescent light. Scale bar, 500 μm. Middle and right: Knockdown of LRRC15 verified by RT-qPCR and western blot analysis. b Knockdown of LRRC15 inhibited ALP activity (left) and ALP staining (right) in MSCs. c LRRC15 knockdown restrained mineralization, shown by Alizarin Red staining (left) and calcium quantitative analysis (middle). Right: LRRC15 knockdown inhibited calcium deposition, shown by Von Kossa staining. d mRNA expression of ALP, OCN, RUNX2, and OSX determined by RT-qPCR, and protein expression of OCN, RUNX2, and OSX determined by western blot analysis. All data shown as mean ± SD, n = 3. *P < 0.05; **P < 0.01. NC negative control cells, LRRC15sh LRRC15 knockdown cells, LRRC15 fifteen-leucine-rich repeat containing membrane protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase, d day, w week, ALP alkaline phosphatase, OCN osteocalcin, RUNX2 runt-related transcription factor 2, OSX osterix

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