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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: LRRC15 promotes osteogenic differentiation of mesenchymal stem cells by modulating p65 cytoplasmic/nuclear translocation

Fig. 5

LRRC15 regulates osteogenic differentiation in a p65-dependent manner. a ALP activity (left), Alizarin Red S staining (middle), and quantification (right) in control, LRRC15 knockdown, and LRRC15 knockdown + BAY11-7082 groups. b RT-qPCR results showing using BAY could enhance downregulated expression of OSX in LRRC15sh cells after osteogenic induction. c Double knockdown of LRRC15 and p65 validated by western blot analysis. d ALP activity (left), ALP staining (middle), and expression of OSX (right) in control, LRRC15 knockdown, and LRRC15/p65 knockdown groups. All data shown as mean ± SD, n = 3. **P < 0.01. d day, w week, NC negative control cells, LRRC15sh LRRC15 knockdown cells, LRRC15 fifteen-leucine-rich repeat containing membrane protein, BAY BAY117082, OSX osterix, GAPDH glyceraldehyde-3-phosphate dehydrogenase

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