Fig. 3From: Transcriptional activator DOT1L putatively regulates human embryonic stem cell differentiation into the cardiac lineageCoexpression of DOT1L and NKX2.5 in (a) KIND1 and (b) HES3 cells during differentiation. Dual immunofluorescence revealed coexpression of histone modifier DOT1L (green, nuclear) along with the master cardiac transcription factor NKX2.5 (red, nuclear) on days 12 and 20 during cardiac directed differentiation of KIND1 and HES3. These markers not expressed in undifferentiated cells (D0) of both KIND1 and HES3 cells. Scale bar: 20 μm. D day, DIC differential interference contrastBack to article page