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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells

Fig. 2

Generation of Pax6-positive dorsal neural progenitor cells (NPCs) from human ESCs and iPSCs via double BMP/SMAD inhibition. a Protocol timeline and representative images of differentiation at different stages. b Immunofluorescence staining of passage 5 YH10 NPCs with NPC markers Nestin, PAX6, SOX1, and SOX2, ventral forebrain marker DLX2, and dorsal forebrain marker FOXG1. c Quantification of percentage of Nestin, PAX6, and SOX1-positive cells. Total number of cells quantified by DAPI nuclear staining using ImageJ (n = 6 independent experiments). d Gene expression analysis by qRT-PCR for pluripotency (OCT4, SOX2), mesodermal (Brachyury), endodermal (GATA4), and neural progenitor (Nestin, PAX6, SOX1, and BTG2) markers. Nuclei stained with DAPI. EB embryoid body, NIM neural induction medium, Inh inhibitor, FGF2 fibroblast growth factor 2, hiPSC human induced pluripotent stem cell, PAX6 paired box protein 6, SOX SRY (sex determining region Y) box, DAPI 4,6′-diamino-2-phenylindole, hESC human embryonic stem cell

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