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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Gas5 is an essential lncRNA regulator for self-renewal and pluripotency of mouse embryonic stem cells and induced pluripotent stem cells

Fig. 4

Gas5 maintains the normal proliferation of mESCs under differentiating culture conditions. a,b CCK-8 assay results showed the proliferation rate of control and Gas5 knockdown (KD) mouse embryonic stem cells (mESCs) under differentiating culture conditions. c Competitive growth assay by mixing mESCs stably expressing control (NC)-green fluorescent protein (GFP) or Gas5 KD-GFP mESCs. d PI staining results indicated the cell cycle in Gas5 KD mESCs under differentiating culture conditions. e Annexin V/PI staining showed the apoptotic cells in control and Gas5 KD mESCs under differentiating culture conditions. *P < 0.05, **P < 0.01, t test, n = 3. Error bars represent SEM of the indicated experiment replicates. mLIF mouse leukemia inhibitory factor, WT wild-type

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