Fig. 7

Gas5 interacts with the Tet family and regulates 5hmC in mESCs. a Expression of all three Tets (Tet1, Tet2, and Tet3) in Gas5 knockdown (KD) mouse embryonic stem cells (mESCs) at the protein level. b The Tet family mediated oxidation production, 5hmC levels in Gas5 KD mESCs. c,d Expression of all three Tets in Gas5 KD mESC- and control mESC-formed teratomas. e The binding signal of Tet1 at the Gas5 promoter region in WT and Tet1 KD mESCs. *P < 0.05, **P < 0.01, ***P < 0.001, t test, n = 3. Error bars represent SEM of the indicated experiment replicates. CHIP chromatin immunoprecipitation, GAPDH glyceraldehyde 3-phosphate dehydrogenase, WT wild-type