Fig. 2

Validation of expression status of TBX21 between normal samples and LUAD. a mRNA expression of TBX21 is significantly upregulated in LUAD compared with normal lung in GSE32863 and GSE68465 (***P < 0.0001, **P < 0.01, t-test method). b, c Immunohistochemistry assay shows protein expression of TBX21 is significantly upregulated in LUAD compared with normal lung tissue (***P < 0.001, t-test method). d Expression of TBX21 is significantly downregulated in normal cell line (MRC-5) compared with tumor cell lines (A549 and H1299) examined by quantitative RT-PCR method (bar plot, top panel) and immunoblot analysis (bottom panels). Error bars represent SEM (**P < 0.01, t-test method). LUAD lung adenocarcinoma