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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stem cells and extracellular matrix scaffold promote muscle regeneration by synergistically regulating macrophage polarization toward the M2 phenotype

Fig. 2

Characterization of human umbilical cord mesenchymal stem cells (hUCMSCs). a Flow cytometric analysis of ex-vivo-expanded hUCMSCs revealed positive expression of CD29, CD44, CD90 and CD105, and negative expression of CD31, CD34 and CD45. b Representative percentage of cell markers for identification. c Representative proliferation of single clone of hUCMSCs. d hUCMSCs seeded in plates induced with osteogenic medium for 10 days. Activity of ALP detected by ALP staining. e hUCMSCs cultured in osteogenic inductive conditions for 28 days, mineralized nodules found by Alizarin Red staining. f Cultured hUCMSCs formed Oil Red O-positive lipid cluster following 14 days of adipogenic induction. gj Cell aggregate (g control) positively expressed fibronectin (h), collagen I (i) and integrin-β1 (j). k Arrangement of cells in cell aggregate observed by SEM. l H&E staining showing cell aggregate was dense and contained plenty of cells. n = 6 per group. Scale bar, 100 μm. PE phycoerythrin, CFU-F fibroblastic colony-forming unit, ALP alkaline phosphatase, Con control, SEM scanning electron microscopy, H&E hematoxylin and eosin

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