Fig. 1

Immunohistochemical examination of MSX2, SP3, and SP6 expression in human–mouse chimeric teeth in presence or absence of FGF8 and SHH. A–C Expression of MSX2, SP3, and SP6 in BSA-treated control samples after 5 days in subrenal culture. MSX2 (A) and SP3 (B), but not SP6 (C), are detected in recombinant implants. D–I Expression of MSX2, SP3, and SP6 in human–mouse chimeric tooth germs in presence of FGF8 and SHH at various time points after subrenal culture. MSX2 and SP3 proteins detected in dental epithelium and mesenchyme, while SP6 exclusively present in dental epithelium at 5 days (D–F) and 7 days (F–I) after grafting. All three proteins present in both ameloblasts and odontoblasts at 12 days after grafting (J–l). Scale bar = 50 μm. BSA bovine serum albumin, FGF8 fibroblast growth factor 8, SHH Sonic hedgehog, 5d 5 days, de dental epithelium, dm dental mesenchyme, am ameloblast, dp dental pulp