Fig. 5
From: Efficient induction of functional ameloblasts from human keratinocyte stem cells
Cytodifferentiation and apoptosis of hKSC-derived dental epithelium in human–mouse chimeric teeth. A Immunofluorescence shows expression profiles of amelogenin and ameloblastin in hKSC-derived ameloblasts at various time points in subrenal culture. Amelogenin and ameloblastin expression not detectable until day 9 (a–d), downregulated at day 28 (e, f), and completely silenced at day 30 (g, h), respectively, after subrenal culture. B TUNEL assay shows programmed cell death of hKSC-derived ameloblasts at various time points in subrenal culture. Obvious apoptosis signals not detected until day 21 (a–f), reached maximal level at day 28 (g–h), and quenched at day 30 (j–l), respectively, after subrenal culture. Scale bar = 100 μm. 8d 8 days, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling, DAPI 4′,6-diamidino-2-phenylindole