Fig. 6

The effect of mesenchymal stem cells (MSCs) cultured in different expansion media on cytokine production from lipopolysaccharide (LPS)-stimulated monocytes. Tumor necrosis factor-α (TNF-α) expression from LPS-stimulated equine monocytes alone or following the addition of MSCs (n = 3) cultured in fetal bovine serum (FBS) or equine platelet lysate (ePL) 6 and 18 h following incubation. Unstimulated monocytes (n = 3) were used as negative control (Mono). No effect of MSCs was seen on the production levels of TNF-α 6 h following their addition to LPS-stimulated monocytes. A statistically significant decrease in TNF-α was detected when MSCs grown in FBS or ePL were added to LPS-stimulated monocytes 18 h following incubation. Regardless of the expansion media, MSCs retain their ability to modulate the production of proinflammatory cytokines from LPS-stimulated monocytes. Date are shown as mean ± standard deviation; n = 3. *P < 0.05 #P < 0.05; statistically significant from all other groups