Fig. 5
From: Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells
AAP antagonizes the hypoxia-mimetic state caused by DFO. a Western blot. Proteins were extracted after 2 days of culturing in media containing the indicated concentrations of DFO, with/without 1 mM AAP. b Effect of DFO and AAP on OCR. The OCR was measured using a flux analyzer before and after the administration of oligomycin (added before cycle 4), FCCP (added before cycle 7), antimycin A, and rotenone (added before cycle 10). c Basal respiration at cycle 1. d Proton leakage at cycle 4. e Maximal respiration at cycle 7. f Non-mitochondrial respiration at cycle 10