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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: The differentiation of mesenchymal stem cells to vascular cells regulated by the HMGB1/RAGE axis: its application in cell therapy for transplant arteriosclerosis

Fig. 1

The proliferation and migration of mesenchymal stem cells (MSCs) in response to high mobility group box 1 (HMGB1) stimulation and receptor for advanced glycation end-product (RAGE) inhibition. a In CCK8 assay, cell survival was decreased after exogenous HMGB1 treatment and pLV-HMGB1 transfection. However, the cells proliferated with resistance to 100 ng/ml HMGB1 after being transfected with pshRNA-RAGE1, pshRNA-RAGE2, and pshRNA-RAGE3. b Transwell assay revealed that cell migration was increased after exogenous HMGB1 treatment and pLV-HMGB1 transfection. However, HMGB1-induced cell migration was inhibited by transfection with pshRNA-RAGE1, pshRNA-RAGE2, and pshRNA-RAGE3. CCK-8 and transwell assay were repeated three and five times, respectively, for each group. Group comparisons were made using the Mann-Whitney test. **P < 0.05

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