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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: The differentiation of mesenchymal stem cells to vascular cells regulated by the HMGB1/RAGE axis: its application in cell therapy for transplant arteriosclerosis

Fig. 6

Cell migration to the graft vessels traced by fluorescent protein and Flag tagging. a The fluorescent protein-labeled cells (marked with white arrows) were visible in the hyperplastic neointima after MSC transplantation. Five random high-power fields were examined in the sections of graft vessels for each rat, and the neointimal cell number was counted to determine the percentage of fluorescent protein-labeled cells in the total cell population. The average percentage was calculated from eight rats for each group. The percentage of neointimal green fluorescent protein (GFP)+ cells was higher in the pLV-HMGB1 group than the pLV-control group. However, the percentage of cherry red fluorescent protein (RFP)+ cells was significantly lower in the pshRNA-RAGE1 and pshRNA-RAGE3 groups than in the pshRNA-control group. Group comparisons were made using the Mann-Whitney test. **P < 0.05. b,c Cell migration to the neointima was confirmed by immunohistochemistry and Western blot with antibody to Flag-tag in the pLV-HMGB1 group, since Flag-tagged HMGB1 was expressed in the cells transfected with pLV-HMGB1 (marked with black arrows). The view of the graft section represents the results from eight rats (b). Homogenate of graft vessels from the same group (eight rats for each group, 50-mg graft vessels for each rat) was prepared for Western blot analysis (c). DAPI 4′,6-diamidino-2-phenylindole, HMGB1 high mobility group box 1, RAGE receptor for advanced glycation end-product

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