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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells

Fig. 3

MeCP2 mediated EPC dysfunction. a, b MeCP2 induced EPC angiogenesis reduction (×40, total tubes used to evaluate tube formation ability). c, d Flow cytometry shows MeCP2 overexpression promoted cell apoptosis (percentage of apoptotic cells with PE Annexin V-positive expression used to assess apoptosis). e, f, TUNEL fluorescence staining shows MeCP2 increased the number of apoptosis cells. gj MeCP2 reduced angiogenesis relative protein levels, p-eNOS/eNOS (g, h) and VEGF (i, j). k, l Protein levels of SIRT1 with MeCP2 overexpression or silence. m, n Protein levels of PGC-1α with MeCP2 overexpression or knockout. oq MeCP2 regulated apoptosis relative proteins. Data reported as mean ± SD, n = 3. *P < 0.05 vs GFP. GFP green fluorescent protein, MeCP2 methyl-CpG-binding protein 2, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling, DAPI 4',6-diamidino-2-phenylindole, eNOS endothelial nitric oxide synthase, p-eNOS phospohorylated eNOS, SIRT1 sirtuin 1, GAPDH glyceraldehyde 3-phosphate dehydrogenase

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