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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Stem cell properties of human clonal salivary gland stem cells are enhanced by three-dimensional priming culture in nanofibrous microwells

Fig. 2

Effects of 3D priming culture on stemness and paracrine secretion. a Heat map showing differential expression of genes in microarrays of monolayer-cultured SGSCs (SGSCs2D) and 3D spheroid-derived SGSCs (SGSCs3D). DEGs (> 2-fold) quantified and antibody reactivity intensity indicated as red pixels (values > 2-fold) and green pixels (values < 2-fold). Stem cell-related genes (b), growth factor genes (c), and WNT-β-catenin-related genes (d, e) in SGSCs3D after whole-genome microarray analysis compared with SGSCs2D. f Confirmation of microarray results for stem cell markers and WNT-β-catenin-related markers after real-time PCR. Data from three independent experiments analyzed and presented as mean ± SEM (n = 3). One-way ANOVA; Tukey’s post hoc test. *Compared with SGSCs2D. *P < 0.05, **P < 0.01, ***P < 0.001. SGSC salivary gland-resident stem cell, EGF epidermal growth factor, HGF hepatocyte growth factor, IGF insulin growth factor, VEGF vascular endothelial growth factor, BDNF brain-derived neurotrophic factor, GDNF glial cell line-derived neurotrophic factor

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