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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells

Fig. 2

Effects of membrane cholesterol perturbation on CAV-1 mRNA expression. a Untreated, cholesterol-depleted MSCs (MβCD), and cholesterol-enriched MSCs (Chol) groups, and b control siRNA (si Ctrl) and caveolin-1 siRNA (si CAV-1) treated groups. RNA was extracted from freshly collected MSCs of the five experimental groups and the CAV-1 mRNA level was assessed by real time reverse transcription PCR. The data represent the mean and 95% confidence intervals of the fold-change relative to the untreated or si Ctrl group; n = 9. These results showed that CAV-1 mRNA expression was affected by the membrane cholesterol level and confirmed effective siRNA-mediated knockdown of CAV-1 gene expression in MSCs. *p < 0.005, **p < 0.0001, versus untreated or si Ctrl group

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