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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Influence of cholesterol/caveolin-1/caveolae homeostasis on membrane properties and substrate adhesion characteristics of adult human mesenchymal stem cells

Fig. 5

Effects of cholesterol and caveolin-1 (CAV-1) perturbation on membrane fluidity. FACS analysis based on the relative fluorescence intensities of excimer and monomer to assess membrane fluidity was performed on the five MSC experimental groups: untreated MSCs, cholesterol-depleted MSCs (MβCD), cholesterol-enriched MSCs (Chol), MSCs transfected with control, nonspecific siRNA (si Ctrl), and MSCs transfected with CAV-1 siRNA (si CAV-1). Results are presented as mean ± SD; n ≥ 3 independent experiments per cell line. The findings show that membrane fluidity is inversely related to membrane cholesterol content, and dependent on the presence of CAV-1. *p < 0.05, **p < 0.001, versus untreated group; #p < 0.05, versus si Ctrl group

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