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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors

Fig. 1

Generation of human pluripotent stem cell (hPSC)-derived multipotent pancreatic progenitors (MPCs). a Schematic summary of the protocols used to generate MPCs from hPSCs. hPSCs were differentiated through four stages, including definitive endoderm (DE), primitive gut tube (PGT), pancreatic foregut (PFG), and MPCs. b RT-PCR analysis of MPCs derived from human embryonic stem cells (hESCs) and induced hPSCs (hiPSCs) at the end of stage 4 (S4) using protocol 1 showing the expression of the MPC and early endocrine progenitor markers (PDX1, NKX6.1, SOX9, NKX2.2, GATA6, PAX6, NGN3, and HNF6). +RT indicates samples with reverse transcriptase, and –RT indicates samples without reverse transcriptase. c Real-time PCR analysis of MPCs differentiated using protocol 1 and protocol 2. Differential gene expression analysis was performed for the main MPC genes (PDX1, NKX6.1, and SOX9). Fold-expression for each TF obtained for protocol 2 was normalized to that of protocol 1. d Flow cytometry analysis for the expression of NKX6.1 at the end of stage 4 of differentiation. All data shown are representative results from at least three independent experiments

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