Fig. 2

Differentiation of hPSC-derived definitive endoderm into different populations of MPCs using protocol 1. Immunofluorescence images of hPSC-derived MPCs showing expression of PDX1 (green) and NKX6.1 (red). MPCs generated from human embryonic stem cells (hESCs) (H1) (a–c) and human induced pluripotent stem cells (hiPSCs) (IMRI90) (d–f) showing three types of MPCs: a, d co-localization of PDX1 and NKX6.1 in MPCs (PDX1⁺/NKX6.1⁺), b, e expression of PDX1 in the absence of NKX6.1 (PDX1⁺/NKX6.1⁻), and c, f appearance of a novel NKX6.1 population lacking PDX1 expression (PDX1⁻/NKX6.1⁺). Squares indicate cells magnified in the insets to the right (a, c, d, f). Note clear distinction between the PDX1⁻/NKX6.1⁺ population and the other populations (PDX1⁺/NKX6.1⁺ and PDX1⁺/NKX6.1⁻). Arrowheads in (a, d) indicate PDX1⁺/NKX6.1⁺ cells and in (c, f) indicate PDX1⁻/NKX6.1⁺ cells. All data shown are representative results from at least three independent experiments. Scale bars = 50 μm