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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors

Fig. 5

Characterization of ductal markers in hPSC-derived MPCs. a Immunofluorescence images of hPSC-derived MPCs showing the expression of SOX9 (green) and NKX2.2 (red) in 3D aggregates generated using protocol 2. Note the expression of SOX9 in the 3D aggregates. b, c Immunofluorescence images of hPSC-derived MPCs showing expression of cytokeratin 19 (green) and NKX6.1 (red). The NKX6.1 expression was noticed in the cells adjacent to cytokeratin-positive cells in a monolayer arrangement of pancreatic progenitors (b); however, cytokeratin 19 was completely absent in the 3D aggregates which were positive for NKX6.1 (arrowheads) (c). All data shown are representative results from at least three independent experiments. Scale bars (a, c) = 100 μm and (b) = 50 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512