Table 2 The details of the primary antibodies used for immunostaining
Antibody | Company | Catalog no. | Dilution |
|---|---|---|---|
Rabbit anti-Chromogranin A | ThermoFisher Scientific | MA5-14536 | 1:4000 |
Rabbit anti-FOXA2 | Cell Signaling Technology | 3143 | 1:500 |
Rabbit anti-OCT4 | Cell Signaling Technology | C30A3 | 1:500 |
Mouse anti-NKX6.1 | DSHB | F55A12 | 1:2000 |
Mouse anti-NKX2.2 | DSHB | 74.5A5 | 1:2000 |
Guinea pig anti-PDX1 | Abcam | Ab47308 | 1:1000 |
Mouse anti-SOX2 | Cell Signaling Technology | L1D6A2 | 1:500 |
Mouse anti-SOX17 | OriGene Technologies | CF500096 | 1:2000 |
Goat anti-NKX6.1 | LifeSpan Biosciences | LS-C124275 | 1:2000 |
Goat anti-SOX9 | R&D Systems | AF3075 | 1:500 |
Sheep anti-NGN3 | R&D Systems | AF3444 | 1:1000 |
Mouse anti-cytokeratin 19 | Merck Millipore | CBL198 | 1:500 |