Fig. 2

Changes in microtubule mechanical status regulates adipogenesis. a Oil Red O staining for triglyceride content in hASCs after 14 days of differentiation treated with nocodazole (0.1 μg/ml), taxol (10 nM), and adipogenic induction medium (AIM) only. Scale bar = 100 μm. b Intracellular triglyceride levels after 14 days of differentiation. Triglyceride content was normalized to total protein content and the control (AIM). One-way ANOVA was performed: *P < 0.05, ***P < 0.001. c Size of lipid droplets after 2 weeks of adipogenesis (n > 30 cells per condition). d The levels of C/EBPα and PPARγ were apparently increased in hASCs with nocodazole (0.1 μg/ml) (AIM+Noco) compared with the control group (AIM) at day 14 after adipogenic cocktail treatment. The taxol group (AIM+Taxol) showed the opposite results. e–h Western blotting and qPCR analysis of C/EBPα and PPARγ in hASCs from the AIM+Noco, AIM+Taxol, and AIM groups after 14 days. i hASCs at 14 days after adipogenic cocktail treatment or with nocodazole (0.1 μg/ml) or taxol (10 nM) were subjected to immunofluorescence staining of α-tubulin (red) and 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bars = 10 μm