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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Cross-talk between microtubules and the linker of nucleoskeleton complex plays a critical role in the adipogenesis of human adipose-derived stem cells

Fig. 4

LINC changes during adipocyte differentiation. a, b hASCs at selected time points were fixed, stained with an anti-Nesprin-1 (green) antibody and/or for DNA (4′,6-diamidino-2-phenylindole (DAPI); blue), and imaged on a confocal microscope. Scale bars = 10 μm. The graph shows average Nesprin-1 (green) intensities derived from three independent differentiation experiments. n > 7 cells; ***P < 0.001, one-way ANOVA. c Nesprin-1 mRNA expression in hASCs at different days postinduction of adipogenesis was quantified by RT-qPCR; P > 0.05, one-way ANOVA. d, e hASCs at selected time points were fixed, stained with the anti-Nesprin-3 antibody and imaged on a confocal microscope. Scale bars = 10 μm. The graph shows average Nesprin-3 (green) intensities derived from three independent differentiation experiments. n > 7 cells; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA. f Nesprin-3 mRNA expression in hASCs at different days postinduction of adipogenesis was quantified by RT-qPCR; *P < 0.05, **P < 0.01, one-way ANOVA. g, h Whole-cell lysates of differentiation-induced hASCs were submitted to Western blotting and probed with an anti-Nesprin-3 antibody. Anti-GAPDH was used to ensure equal loading. The graph shows the average Nesprin-3 band intensities normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) derived from three independent differentiation experiments; *P < 0.05, ** P < 0.01, ***P < 0.001, one-way ANOVA

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