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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Cross-talk between microtubules and the linker of nucleoskeleton complex plays a critical role in the adipogenesis of human adipose-derived stem cells

Fig. 5

Expression of SUN2 is increased during adipogenesis. a SUN2 mRNA expression in hASCs at different days postinduction of adipogenesis was quantified by RT-qPCR; **P < 0.01, ***P < 0.001, one-way ANOVA. b, c Whole-cell lysates of differentiation-induced hASCs were submitted to Western blotting and probed with anti-SUN2 antibody; **P < 0.01, ***P < 0.001, one-way ANOVA. d SUN2 mRNA expression in hASCs with nocodazole (Noco; 0.1 μg/ml) and taxol (10 nM) after 4 days of adipogenesis was quantified by RT-qPCR; **P < 0.01, ***P < 0.001, one-way ANOVA. e hASCs at selected time points were fixed, stained with the anti-SUN2 antibody, and imaged on a confocal microscope. Scale bars = 20 μm. AIM, adipogenic induction medium; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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Stem Cell Research & Therapy

ISSN: 1757-6512