Fig. 6

Knockdown of SUN2 inhibits adipogenic differentiation (AD) of hASCs by downregulating adipogenesis-promoting signaling pathway PPARγ and overexpressing microtubules (MTs). a RT-qPCR to determine knockdown efficiency in RNA samples; ***P < 0.001). b, c Oil Red O staining for triglyceride content in transfected hASCs after 7 days of differentiation. Scale bar = 100 μm. Intracellular triglyceride levels at day 7 of differentiation. Triglyceride content was normalized to total protein content and the control. Green fluorescent protein (GFP) represents transfected cells. An unpaired Student’s t test was performed; **P < 0.01. d, e hASC lysates of cells transfected with the indicated small interfering (si)RNAs were submitted to Western blotting and probed with an anti-SUN2 antibody and for the adipogenesis markers C/EBPα and PPARγ. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used to ensure equal loading and normalized α-tubulin, C/EBPα, and PPARγ2 protein levels. GM, growth medium