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Table 2 SYBR green primers used for PCR analyses

From: Inflammatory licensed equine MSCs are chondroprotective and exhibit enhanced immunomodulation in an inflammatory environment

Gene

Abbreviation

Function

Forward 5′–3’

Reverse 5′–3’

Secretory carrier membrane protein 3

SCAMP3

Housekeeping gene

CTGTGCTGGGAATTGTGATG

ATTCTTGCTGGGCCTTCTG

Major histocompatibility complex class I

MHC-I

Self-recognition

ACCGTGAGGTCACCCTGA

CTCCGTGTCCTGGGTCA

Major histocompatibility complex class II

MHC-II

Antigen presentation

TCCCTATGCTGGGACTTTTC

CGCCAGGCTTCAGATAGAAC

Indoleamine 2,3-dioxygenase

IDO

Mediator of immunomodulation

TCATGACTACGTGGACCCAAAA

CGCCTTCATAGAGCAGACCTTC

Prostaglandin endoperoxide synthase 2

PTGS2

Mediator of immunomodulation

CAGCATAAACTGCGCCTTTTC

AGGCGGGTAGATCATTTCCA

Inducible nitric oxide synthase

NOS2

Mediator of immunomodulation

CCAACAATGGCAACATCAGGT

TGAGCATTCCAGATCCGGA

Interleukin-6

IL-6

Cytokine

TGCTGGCTAAGCTGCATTCA

GGAAATCCTCAAGGCTTCGAA

Interleukin-8

IL-8

Cytokine

GCTGGCTGTTGCTCTCTTG

CCGAAGCTCTGCAGTAATTCTT

Interleukin-10

IL-10

Cytokine

GCCTTGTCGGAGATGATCCA

TTTTCCCCCAGGGAGTTCAC

Tumor necrosis factor alpha

TNF-α

Cytokine

AAAGGACATCATGAGCACTGAAAG

GGGCCCCCTGCCTTCT

Interferon gamma

IFN-γ

Cytokine

CCAGCGCAAAGCAATAAGTG

GGCCTCGAAACGGATTCTG

C-C motif chemokine 2

CCL2

Chemokine

GGCTCAGCCAGATGCAATTA

GCTTTCTTGTCCAGCTGCTT

C-X-C motif chemokine 10

CXCL10

Chemokine

GACTCTGAGTGGAACTCAAGGAAT

GTGGCAATGATCTCAACACG

Collagen type 2

COL2A1

Cartilage extracellular matrix

TCAAGTCCCTCAACAACCAGATC

GTCAATCCAGTAGTCTCCGCTCTT