Fig. 1

Intraperitoneally infused cAT-MSC-secreted TSG-6 plays an essential role in alleviating IBD. Dextran sulfate sodium (DSS)-induced colitis mice were administered with canine adipose tissue-derived mesenchymal stem cells (cAT-MSCs) transfected with tumor necrosis factor-α-induced gene/protein-6 (TSG-6) small interfering (si)RNA (siTSG6-cAT-MSC), cAT-MSCs transfected with scrambled siRNA (siCTL-cAT-MSC), naive cAT-MSCs, or phosphate-buffered saline (PBS; vehicle control) on day 1. a Body weight was measured every day and expressed in terms of the relative change from the weight measured on day 0. Mice were sacrificed on day 10 and b Disease Activity Index (DAI) and c colon length were assessed. d Representative hematoxylin and eosin staining of the colon tissue sections and their histological scores are shown. Scale bars = 100 μm. Four to six mice per group were used. Results are shown as the mean ± standard deviation. **P < 0.01, ***P < 0.001. ns, not significant