Fig. 1
Effect of BMC transplantation into skeletal muscle of ALS transgenic SOD1G93A mice. a Representative longitudinal section of quadriceps femoris muscle showing immunohistochemical labeling for GFP cells (green). Nuclei stained with DAPI (blue). b Effect of BMC transplant on course of disease in SOD1G93A mice. BMC-treated mice are shown in dark gray and sham-injected mice in light gray. RT-PCR amplification for the detection of GFP expression in mouse quadriceps femoris after intramuscular BMC transplantation (T treated mice, P GFP cells used as positive control, B Black control, M RNA Marker). c Onset of symptoms and mortality of BMC-treated and sham-injected mice presented by days. d, e Cumulative probability of d onset of disease symptoms and e survival. f, g Motor functions assessed by rotarod and treadmill tests. f Latency of fall when submitted to rotarod test. g Maximum speed attained as measured on the treadmill. n = 20 sex-balanced animals per group. ^p < 0.10; *p < 0.05. Error bars indicate SEM. BMC bone marrow cell