Table 1 Primer sequences used for gene expression analysis
Gene | 5′ → 3′ | Primer sequence |
|---|---|---|
NT4 | Forward | TGAGCTGGCAGTATGCGAC |
Reverse | CAGCGCGTCTCGAAGAAGT | |
NGF | Forward | GCACTACACCCATCAAGTTCA |
Reverse | TCCTGAGTCATGCTCACAAGT | |
BDNF | Forward | TCATACTTCGGTTGCATGAAGG |
Reverse | GTCCGTGGACGTTTACTTCTTT | |
NT3 | Forward | AGTTTGCCGGAAGACTCTCTC |
Reverse | GGGTGCTCTGGTAATTTTCCTTA | |
GDNF | Forward | CGCCGGTAAGAGGCTTCTC |
Reverse | CGTCATCAAACTGGTCAGGATAA | |
VEGF | Forward | GCCAGACAGGGTTGCCATAC |
Reverse | GGAGTGGGATGGATGATGTCAG | |
IGF1 | Forward | CTGGACCAGAGACCCTTTGC |
Reverse | GGACGGGGACTTCTGAGTCTT | |
EGF | Forward | AGCATCTCTCGGATTGACCCA |
Reverse | CCTGTCCCGTTAAGGAAAACTCT | |
GAPDH | Forward | AGGTCGGTGTGAACGGATTTG |
Reverse | GGGGTCGTTGATGGCAACA | |
β-actin | Forward | AGAGGGAAATCGTGCGTGAC |
Reverse | CAATAGTGATGACCTGGCCGT | |
GFP | Forward | CTG CTG CCC GAC AAC CA |
Reverse | GAA CTC CAG CAG GAC GAC CAT GTG |