Fig. 7

Flow cytometric analysis of the indicated inflammatory molecules by natural killer (NK) cells harvested from NK/DPMSC cytolytic experiments. Decidua parietalis mesenchymal stem/stromal cells (DPMSCs) significantly increased NK cell expression of the interleukin-18 receptors IL-18Rα (c) and IL-18Rβ (d), and Toll-like receptor (TLR)7 (h), while the expression of TLR3 (g) and TLR (i) was significantly decreased. DPMSCs had no significant effect on NK cell expression of tumor necrosis factor (TNF)-α (a), IL-12R (b), interferon (IFN)-γR1 (e), and IFN-γR2 (f) (P > 0.05). The levels of the expression of activating and inhibitory receptors in NK cells were recorded as median fluorescence intensity (MFI) as determined by flow cytometry. Experiments were carried out in triplicate and repeated 10 times using NK cells and DPMSCs prepared from the peripheral blood of 10 different healthy donors and 10 different fetal membranes, respectively. Bars represent standard errors. *P < 0.05, versus untreated NK cells