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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages

Fig. 6

Validation of microarray results by qRT-PCR and western blot analysis. a qRT-PCR testing expression of selected TF-encoding mRNAs using the same total RNA as analyzed by microarray. Data for OVs, RGCs and RPE cells presented as fold-changes relative to mRNA level in hiPSC, standard deviation between replicates shown as error bars. b qRT-PCR testing expression of selected TF-encoding mRNAs using RNA obtained from independent set of cells: hiPSCs, RGCs after 9 days of differentiation from OVs (RGC 9), RGCs after 17 days of differentiation from OVs (RGC 17), hiPSC-derived RPE cells (RPE) and RPE cell line ARPE-19 (ARPE19). Data presented as fold-changes relative to hiPSC. c Western blot analysis demonstrating overexpression of EBF1 and EBF3 TFs in RGCs, but not in RPE cells and hiPSCs, similarly to well-known RGC markers SNCG and ISL1. iPSC induced pluripotent stem cell, RGC retinal ganglion cell, RPE retinal pigment epithelium

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