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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages

Fig. 7

Effect of knockdown of EBF1 on differentiation of RGCs. a Western blot analysis demonstrating expression levels of EBF1 and indicated RGC markers in RGCs differentiated from control and EBF1 knockdown (EBF KD1 and EBF KD2) hiPSCs. GAPDH used as loading control. b Immunofluorescent microscopy showing expression of EBF1, BRN3A and Tuj1 in control and EBF1 knockdown RGCs (EBF1 KD2). c Bright-field microscopy demonstrating morphology of OVs (top) and RGCs (bottom) derived from control and EBF1 knockdown (EBF1 KD2) hiPSCs. Arrows indicate neural retina precursor layer of brighter color. DAPI 4′,6-diamidino-2-phenylindole, OV optic vesicle, RGC retinal ganglion cell

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Stem Cell Research & Therapy

ISSN: 1757-6512